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syngene® genetools gel analysis software (Syngene)

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Syngene syngene® genetools gel analysis software
Syngene® Genetools Gel Analysis Software, supplied by Syngene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syngene® genetools gel analysis software/product/Syngene
Average 90 stars, based on 1 article reviews

syngene® genetools gel analysis software - by Bioz Stars, 2026-03

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Characterization of JEV, YFV and CHKV protein expression and VLP release. (A) 293 T cells were transfected with JEV CprME expression vector. Cells were analyzed for E protein and Capsid protein expression after staining with respective antibodies followed by fluorescence microscopy. (B) 293 T cells were transfected with JEV CprME expression vector alone or the JEV CprME vector along with Zika NS2B-3 expression plasmid. Culture supernatants were ultracentrifuged and analyzed for E protein and Capsid protein expression by western blotting. (C) 293 T cells were transfected with YFV CprME expression vector and analyzed for E protein and Capsid protein expression by immunofluorescence microscopy. (D) 293 T cells were transfected with YFV CprME expression vector alone or the YFV CprME vector along with Zika NS2B-3 expression plasmid. Culture supernatants were ultracentrifuged and analyzed for E protein and Capsid protein expression by western blotting. (E) 293 T cells were transfected with CHKV expression vector and analyzed for E1-E2 protein expression by immunofluorescence microscopy. (F) 293 T cells were transfected with CHKV expression vector. Culture supernatants were ultracentrifuged and analyzed for E1-E2 protein expression by western blotting. Images A, C and E were analyzed using NIS Elements AR software version 3.2 (Nikon; https://nis-elements-viewer.software.informer.com/3.2/ ). Images B, D and F were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Journal: Scientific Reports

Article Title: Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus

doi: 10.1038/s41598-020-61103-1

Figure Lengend Snippet: Characterization of JEV, YFV and CHKV protein expression and VLP release. (A) 293 T cells were transfected with JEV CprME expression vector. Cells were analyzed for E protein and Capsid protein expression after staining with respective antibodies followed by fluorescence microscopy. (B) 293 T cells were transfected with JEV CprME expression vector alone or the JEV CprME vector along with Zika NS2B-3 expression plasmid. Culture supernatants were ultracentrifuged and analyzed for E protein and Capsid protein expression by western blotting. (C) 293 T cells were transfected with YFV CprME expression vector and analyzed for E protein and Capsid protein expression by immunofluorescence microscopy. (D) 293 T cells were transfected with YFV CprME expression vector alone or the YFV CprME vector along with Zika NS2B-3 expression plasmid. Culture supernatants were ultracentrifuged and analyzed for E protein and Capsid protein expression by western blotting. (E) 293 T cells were transfected with CHKV expression vector and analyzed for E1-E2 protein expression by immunofluorescence microscopy. (F) 293 T cells were transfected with CHKV expression vector. Culture supernatants were ultracentrifuged and analyzed for E1-E2 protein expression by western blotting. Images A, C and E were analyzed using NIS Elements AR software version 3.2 (Nikon; https://nis-elements-viewer.software.informer.com/3.2/ ). Images B, D and F were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Article Snippet: Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Techniques: Expressing, Transfection, Plasmid Preparation, Staining, Fluorescence, Microscopy, Western Blot, Immunofluorescence, Software

Generation and characterization of bicistronic lentiviral vectors expressing flaviviral structural proteins. (A) JEV CprME was cloned into a lentiviral vector that included an IRES sequence followed by the Zika NS2B-3 protease. 293 T cells were transfected with the JEV CprME construct alone or the bicistronic JEV lentiviral construct. Culture supernatants were harvested and VLPs analyzed for JEV E protein and Capsid protein secretion via western blotting. (B) YFV CprME was cloned into a lentiviral vector as above and VLP secretion determined in the culture supernatants by western blotting for E and Capsid protein. (C) CHIKV C-E3-E2-E1 genes were cloned into a lentiviral vector and VLP secretion determined in the culture supernatants by western blotting for E1-E2 protein. Images were analyzed using GENETOOLS gel analysis Software version 4.03 (f). (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Journal: Scientific Reports

Article Title: Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus

doi: 10.1038/s41598-020-61103-1

Figure Lengend Snippet: Generation and characterization of bicistronic lentiviral vectors expressing flaviviral structural proteins. (A) JEV CprME was cloned into a lentiviral vector that included an IRES sequence followed by the Zika NS2B-3 protease. 293 T cells were transfected with the JEV CprME construct alone or the bicistronic JEV lentiviral construct. Culture supernatants were harvested and VLPs analyzed for JEV E protein and Capsid protein secretion via western blotting. (B) YFV CprME was cloned into a lentiviral vector as above and VLP secretion determined in the culture supernatants by western blotting for E and Capsid protein. (C) CHIKV C-E3-E2-E1 genes were cloned into a lentiviral vector and VLP secretion determined in the culture supernatants by western blotting for E1-E2 protein. Images were analyzed using GENETOOLS gel analysis Software version 4.03 (f). (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Article Snippet: Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Techniques: Expressing, Clone Assay, Plasmid Preparation, Sequencing, Transfection, Construct, Western Blot, Software

Generation of stable cell lines secreting JEV, YFV and CHIKV VLPs. 293 T cells were transduced with (A) JEV (B) YFV or (C) CHKV structural protein containing lentivirus particles. Transduced cells were bulk selected with blasticidin followed by limiting dilution single cell cloning. For each virus, several single cell clones were characterized for Envelope protein staining on the cell surface via flow cytometry and VLP secretion into the culture supernatants via western blotting. Mean Fluorescence intensity (MFI) of Envelope protein staining for the different single cell clones for JEV, YFV and CHIKV is shown on the right. Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ). Flow cytometry histograms were created using the FloJo Software version 10.6.0 (Tree Star, https://www.flowjo.com/solutions/flowjo/downloads ).

Journal: Scientific Reports

Article Title: Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus

doi: 10.1038/s41598-020-61103-1

Figure Lengend Snippet: Generation of stable cell lines secreting JEV, YFV and CHIKV VLPs. 293 T cells were transduced with (A) JEV (B) YFV or (C) CHKV structural protein containing lentivirus particles. Transduced cells were bulk selected with blasticidin followed by limiting dilution single cell cloning. For each virus, several single cell clones were characterized for Envelope protein staining on the cell surface via flow cytometry and VLP secretion into the culture supernatants via western blotting. Mean Fluorescence intensity (MFI) of Envelope protein staining for the different single cell clones for JEV, YFV and CHIKV is shown on the right. Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ). Flow cytometry histograms were created using the FloJo Software version 10.6.0 (Tree Star, https://www.flowjo.com/solutions/flowjo/downloads ).

Article Snippet: Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Techniques: Stable Transfection, Transduction, Clone Assay, Staining, Flow Cytometry, Western Blot, Fluorescence, Software

Characterization of stable single cell clones. (A) JEV-JD12, YFV-YF9 and CHIKV-CH3 cell lines were stained for E and/or Capsid protein expression. Cells were analyzed by fluorescence microscopy and images acquired. (B) The stable cell lines were cultured in the presence (+) or absence (−) of blasticidin for a period of 30 days with routine sub-culturing. Culture supernatants were analyzed for E protein expression by western blotting. M = Protein molecular weight marker. (C) VLPs harvested from the individual stable cell lines were negatively stained and analyzed by Transmission Electron Microscopy. Scale = 50 nm. Fluorescence microscopy images were analyzed using NIS Elements AR software version 3.2 (Nikon; https://nis-elements-viewer.software.informer.com/3.2/ ). Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Journal: Scientific Reports

Article Title: Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus

doi: 10.1038/s41598-020-61103-1

Figure Lengend Snippet: Characterization of stable single cell clones. (A) JEV-JD12, YFV-YF9 and CHIKV-CH3 cell lines were stained for E and/or Capsid protein expression. Cells were analyzed by fluorescence microscopy and images acquired. (B) The stable cell lines were cultured in the presence (+) or absence (−) of blasticidin for a period of 30 days with routine sub-culturing. Culture supernatants were analyzed for E protein expression by western blotting. M = Protein molecular weight marker. (C) VLPs harvested from the individual stable cell lines were negatively stained and analyzed by Transmission Electron Microscopy. Scale = 50 nm. Fluorescence microscopy images were analyzed using NIS Elements AR software version 3.2 (Nikon; https://nis-elements-viewer.software.informer.com/3.2/ ). Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Article Snippet: Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Techniques: Clone Assay, Staining, Expressing, Fluorescence, Microscopy, Stable Transfection, Cell Culture, Western Blot, Molecular Weight, Marker, Transmission Assay, Electron Microscopy, Software

Adaptation of stable cell lines for growth in suspension culture. (A) JEV, YFV, ZIKV and CHIKV stable cell lines were adapted to grow in suspension culture. After complete adaptation, cells were stained for E protein expression via flow cytometry. The respective adherent counterparts were used as staining controls alongside. (B) VLP production from the adapted suspension cell lines was determined by western blotting for the E protein and compared to VLP secretion from the respective adherent counterparts. (C) Morphology of suspension cell lines via brightfield microscopy and comparison with their respective adherent counterparts. Flow cytometry histograms were created using the FloJo Software version 10.6.0 (Tree Star, https://www.flowjo.com/solutions/flowjo/downloads ). Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ). Bright field images were analyzed using NIS Elements AR software version 3.2 (Nikon, https://nis-elements-viewer.software.informer.com/3.2/ ).

Journal: Scientific Reports

Article Title: Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus

doi: 10.1038/s41598-020-61103-1

Figure Lengend Snippet: Adaptation of stable cell lines for growth in suspension culture. (A) JEV, YFV, ZIKV and CHIKV stable cell lines were adapted to grow in suspension culture. After complete adaptation, cells were stained for E protein expression via flow cytometry. The respective adherent counterparts were used as staining controls alongside. (B) VLP production from the adapted suspension cell lines was determined by western blotting for the E protein and compared to VLP secretion from the respective adherent counterparts. (C) Morphology of suspension cell lines via brightfield microscopy and comparison with their respective adherent counterparts. Flow cytometry histograms were created using the FloJo Software version 10.6.0 (Tree Star, https://www.flowjo.com/solutions/flowjo/downloads ). Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ). Bright field images were analyzed using NIS Elements AR software version 3.2 (Nikon, https://nis-elements-viewer.software.informer.com/3.2/ ).

Article Snippet: Gel images were analyzed using GENETOOLS gel analysis Software version 4.03 (f) (Syngene, https://www.syngene.com/software/genetools-automatic-image-analysis/ ).

Techniques: Stable Transfection, Staining, Expressing, Flow Cytometry, Western Blot, Microscopy, Software

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